Wolverine14MG

“Wolverine” (BPC‑157 + TB‑500) is an informal research nickname commonly used for a co‑administration concept that pairs BPC‑157 (a pentadecapeptide investigated for cytoprotective and pro‑healing activity) with TB‑500, a short peptide fragment derived from thymosin β4 (Tβ4 17–23, often N‑acetylated). Composition is not standardized and can vary by source; the most prevalent definition is BPC‑157 + TB‑500 only. This document summarizes identifiers, combined rationale, safety considerations, and literature references for the constituents.

 

Chemical Structure / Identifiers — BPC‑157

Property	Detail
Sequence	Gly‑Glu‑Pro‑Pro‑Pro‑Gly‑Lys‑Pro‑Ala‑Asp‑Asp‑Ala‑Gly‑Leu‑Val (15 aa)
Molecular Formula	C62H98N16O22
Molecular Weight	≈ 1419.55 g/mol
CAS Number	137525‑51‑0
PubChem CID	9941957
Synonyms	Body Protection Compound‑157; PL 14736; bepecin (research name)

 

Chemical Structure / Identifiers — TB‑500 (Tβ4 17–23 fragment)

Property	Detail
Common Name	TB‑500; thymosin β4 17–23 fragment
Parent Protein	Thymosin β4 (TMSB4X), 43 aa
Fragment Sequence	Leu‑Lys‑Lys‑Thr‑Glu‑Thr‑Gln (LKKTETQ); frequently N‑acetylated
Standardization Note	“TB‑500” is a research nickname; exact termini/salt form can vary by source
Registry/DB	NCATS GSRS lists TB‑500 as N‑Acetyl‑Leu‑Lys‑Lys‑Thr‑Glu‑Thr‑Gln (Tβ4 17–23)
Related Entry	Parent Tβ4 (also called timbetasin) is cataloged separately in PubChem

 

Primary Research Focus (Constituents)

• BPC‑157: preclinical evidence for gastrointestinal mucosal protection, tendon/ligament healing, angiogenesis support, and anti‑inflammatory signaling; cell migration and fibroblast outgrowth effects reported.
• TB‑500 / Thymosin β4 domain: actin‑binding motif mapped to repair processes; promotes cell migration, angiogenesis, and wound closure in ocular and dermal models; anti‑inflammatory effects described.

 

Combined Rationale (Research Context)

• Complementary mechanisms: BPC‑157 is reported to be broadly cytoprotective and pro‑angiogenic in animal models, while the Tβ4 17–23 fragment lies within an actin‑binding region linked to cell migration and cytoskeletal remodeling. Pairing can be hypothesized to support parallel phases of tissue repair (hemostasis/inflammation to proliferation/remodeling).
• Evidence base: synergy for the exact BPC‑157 + TB‑500 pairing has not been established in controlled human trials. Most literature evaluates each constituent separately in preclinical models.

 

Safety / Limitations

• Research Use Only; neither BPC‑157 nor TB‑500 is approved by major regulators for treatment or prevention of disease.
• Composition variability: “TB‑500” is not a single standardized entity; identity and purity can differ by supplier. Confirm sequence and quality for any laboratory work.
• Human data are limited; dosing, pharmacokinetics, and long‑term safety remain uncertain.
• Anti‑doping: parent thymosin β4 and related peptides are prohibited by many sports bodies; laboratory use in athletic populations should consider current regulations.

 

Key Publications / References

PubChem Compound Summary: BPC‑157 (CID 9941957). https://pubchem.ncbi.nlm.nih.gov/compound/9941957

Pentadecapeptide BPC‑157 enhances tendon outgrowth and fibroblast migration. J Appl Physiol. 2010. PubMed: https://pubmed.ncbi.nlm.nih.gov/21030672/  PMC overview: https://pmc.ncbi.nlm.nih.gov/articles/PMC6271067/

Gastric pentadecapeptide BPC‑157 accelerates healing of transected rat Achilles tendon. J Orthop Res. 2003. PubMed: https://pubmed.ncbi.nlm.nih.gov/14554208/

Stable gastric pentadecapeptide BPC‑157: review of anti‑ulcer and systemic cytoprotective effects. World J Gastroenterol. 2015. PMC: https://pmc.ncbi.nlm.nih.gov/articles/PMC4717094/

NCATS GSRS substance record: TB‑500 (N‑Acetyl‑Leu‑Lys‑Lys‑Thr‑Glu‑Thr‑Gln; Tβ4 17–23). https://gsrs.ncats.nih.gov/ginas/app/ui/substances/e850a4ce-7777-4d25-ae69-ab7174c798a4

PubChem Compound Summary: Thymosin β4 (timbetasin). https://pubchem.ncbi.nlm.nih.gov/compound/Thymosin-beta4

Progress on the Function and Application of Thymosin β4. 2021 review. PMC: https://pmc.ncbi.nlm.nih.gov/articles/PMC8724243/

Corneal and skin wound healing literature for Tβ4 (review). https://europepmc.org/article/med/20536468

 

Disclaimer: For research background only. Not medical advice. “Wolverine” is an informal term; composition is not standardized, and combined use has not been validated in human trials.